Lyo-Ready qPCR master mix is a glycerol-free formulation that contains Taq polymerase, reaction buffer, dNTP, MgCl2, a passive reference dye and lyo-excipients. It is ideal for developing room temperature, lyophilized, multiplex qPCR diagnostic tests and is suited for high-throughput, automated platforms requiring milli-second rehydration times. It can be used as a wet mix or it can be lyophilized without impacting its performance post rehydration.